Academic

Publications as Associate Professor

2024

• Dual-targets binding protection mediated rolling circle transcription with tandem fluorescent RNA aptamers for label-free detection of liver cancer biomarkers. Guan C, Ma Y, Sun P, Wu Yu, Arroyo-Currás N, Chen G, Feng C. Sens. Actuators B Chem., 2024, DOI: 10.1016/j.snb.2024.135521.

• Rational Approach to Optimizing Conformation-Switching Aptamers for Biosensing Applications. Wolfe M, Cramer A, Webb S, Goorskey E, Chushak Y, Mirau P, Arroyo-Currás N, Chávez J L. ACS Sens., 2024, DOI: 10.1021/acssensors.3c02004.

• Nucleic Acid-based Electrochemical Sensors Facilitate the Study of DNA Binding by Platinum (II)-based Antineoplastics. Wu Y, Arroyo-Currás N. Angew. Chem. Int. Ed., 2024, DOI: 10.1002/anie.202312402.

2023

• Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors. Shaver A, Mallires K, Harris J, Kavner J, Wang B, Gottlieb R, Lión-Villar J, Herranz M A, Martín N, Arroyo-Currás N. ACS Appl. Polym. Mater., 2023, DOI: 10.1021/acsapm.3c02206.

• Analytical Validation of Aptamer-Based Serum Vancomycin Monitoring Relative to Automated Immunoassays. Liu Y, Mack JO, Shojaee M, George A, Clarke W, Patel N, Arroyo-Currás N. ACS Sens., 2023, DOI: 10.1021/acssensors.3c01868. Data access: [Zenodo Repository]

• 3D-Printed, aptamer-based microneedle sensor arrays using magnetic placement on live rats for pharmacokinetic measurements in interstitial fluid. Reynoso M, Chang A-Y, Wu Y, Murray R, Suresh S, Dugas Y, Wang J, Arroyo-Currás N. Biosens. Bioelecton., 2023, DOI: 10.1016/j.bios.2023.115802.

• Implantable Hydrogel-Protective DNA Aptamer-Based Sensor Supports Accurate, Continuous Electrochemical Analysis of Drugs at Multiple Sites in Living Rats. Li S, Dai J, Zhu M, Arroyo-Currás N, Li H, Wang Y, Wang Q, Lou X, Kippin T E, Wang S, Plaxco K W, Li H, Xia F. ACS Nano, 2023, DOI: 10.1021/acsnano.3c06520.

• Stability of N-Heterocyclic Carbene Monolayers under Continuous Voltammetric Interrogation. Pellitero M A, Jensen I M, Dominique N L, Ekowo L C, Camden J P,Jenkins D M, Arroyo-Currás N. ACS Appl. Mater. Interfaces, 2023, DOI: 10.1021/acsami.3c06148. Data access: [JHU Repository]

• High-precision monitoring of and feedback control over drug concentrations in the brains of freely moving rats. Gerson J, Erdal M K, McDonough M H, Ploense K L, Dauphin-Ducharme P, Honeywell K M, Leung K K, Arroyo-Curras N, Gibson J M, Emmons N A, Meiring W, Hespanha J P, Plaxco K W, Kippin T E.  Science advances, 2023, DOI: 10.1126/sciadv.adg3254

• Continuous Molecular Monitoring in the Body via Nucleic Acid–based Electrochemical Sensors: The Need for Statistically-powered Validation. Yuan Y, Arroyo-Currás N, Curr. Opin. Electrochem., 2023, DOI:10.1016/j.coelec.2023.101305

• Perspective—The Feasibility of Continuous Protein Monitoring in Interstitial Fluid. Young T, Clark V, Arroyo-Currás N, Heikenfeld J, ECS Sensors Plus, 2023, DOI:10.1149/2754-2726/accd7e

• Expanding the Monolayer Scope for Nucleic Acid-Based Electrochemical Sensors Beyond Thiols on Gold: Alkylphosphonic Acids on ITO. Shaver, A; Arroyo-Currás, N., ECS Sensors Plus, 2023, DOI:10.1149/2754-2726/acc4d9. Data access: [JHU Repository]

• Explaining the Decay of Nucleic Acid-Based Sensors under Continuous Voltammetric Interrogation. Clark, V.; Pellitero, M.A.; Arroyo-Currás, N., Anal. Chem., 2023, DOI:10.1021/acs.analchem.2c051.
  Data access: [JHU Repository]

Publications as Assistant Professor

2023

• Os(II/III) complex supports pH-insensitive electrochemical DNA-based sensing with superior operational stability than the benchmark methylene blue reporter. Pellitero, M.A.; Kundu, N.; Sczepanski, J.T.; Arroyo-Currás, N., Analyst., 2023, DOI:10.1039/D2AN01901A

2022

• Optimization of Vancomycin Aptamer Sequence Length Increases the Sensitivity of Electrochemical, Aptamer-Based Sensors In Vivo. Shaver, A.; Mahlum, J.D.; Scida, K.; Johnston, M.L.; Pellitero, M. A.; Wu, Y.; Carr, G.V.; Arroyo-Currás, N., ACS Sens., 2022, DOI:10.1021/acssensors.2c01910

• Human Cyclophilin B Nuclease Activity Revealed via Nucleic Acid-Based Electrochemical Sensors. Clark, V.; Waters, K.; Orsburn, B.; Bumpus, N.N.; Kundu, N.; Sczepanski, J.T.; Ray, P.; Arroyo-Currás, N.; Angew. Chem. Int. Ed., 2022, DOI: 10.1002/anie.202211292

• Antibody–Invertase Fusion Protein Enables Quantitative Detection of SARS-CoV-2 Antibodies Using Widely Available Glucometers. Leonard, E. K.; Pellitero, M.A.; Juelg, B.; Spangler, J.B.; Arroyo-Currás, N.; J. Am. Chem. Soc., 2022, DOI: 10.1021/jacs.2c02537

• Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring. Wu, Y.; Tehrani, F.; Teymourian, H.; Mack, J.; Shaver, A.; Reynoso, M.; Kavner, J.; Huang, N.; Furmidge, A.; Duvvuri, A.; Nie, Y.; Laffel, L. M.; Doyle, F.J. III; Patti, M-E.; Dassau, E.; Wang, J.; Arroyo-Currás, N.; Anal. Chem., 2022, DOI: 10.1021/acs.analchem.2c00829

• Study of Surface Modification Strategies to Create Glassy Carbon‑supported, Aptamer‑based Sensors for Continuous Molecular Monitoring. Pellitero, M. A.; Arroyo-Currás, N.; Anal. Bioanal. Chem., 2022, DOI: 10.1007/s00216-022-04015-5

• Electrochemical Aptamer-Based Sensors: A Platform Approach to High-Frequency Molecular Monitoring In Situ in the Living Body. Dauphin-Ducharme, P.; Ploense, K.L.; Arroyo-Currás, N.; Kippin, T.E.; Plaxco, K.W.; In Biomedical Engineering Technologies: Volume 1, Ossandon, M. R.;  Baker, H.; Rasooly, A., Eds. Springer US: New York, NY, 2022; 479-492. DOI: 10.1007/978-1-0716-1803-5_25

2021

• The Challenge of Long-term Stability for Nucleic Acid-based Electrochemical Sensors. Shaver, A.; Arroyo-Curras, N.; Curr. Opin. Electrochem., 2021, DOI: 10.1016/j.coelec.2021.100902
• Nanoscale Bioreceptor Layers Comprising Carboxylated Polythiophene for Organic Electrochemical Transistor-Based Biosensors. Song, Y.; Lamberty, Z.D.; Liang, J.; Pellitero, M.A.; Wagner, J.S.; Jumai’an, E.; Bevan, M.A.; Frechette, J.; Arroyo-Currás, N.; Katz, H. E.; ACS Appl. Nano Mater., 2021, DOI:10.1021/acsanm.1c02949
• Chemical Equilibrium-Based Mechanism for the Electrochemical Reduction of DNA-Bound Methylene Blue Explains Double Redox Waves in Voltammetry. Mahlum, J.D.; Pellitero, M.A.; Arroyo-Currás, N; J. Phys. Chem. C, 2021, DOI: 10.1021/acs.jpcc.1c00336
• Nuclease Hydrolysis Does Not Drive the Rapid Signaling Decay of DNA Aptamer-Based Electrochemical Sensors in Biological Fluids. Shaver, A.; Kundu, N.; Young, B.E.; Vieira, P.A.; Sczepanski, J.T.; Arroyo-Currás, N; Langmuir, 2021, DOI: 10.1021/acs.langmuir.1c00166
• Interrogation of Electrochemical Aptamer-Based Sensors via Peak-to-Peak Separation in Cyclic Voltammetry Improves the Temporal Stability and Batch-to-Batch Variability in Biological Fluids. Pellitero, M.A.; Curtis, S.D.; Arroyo-Curras, N.; ACS Sensors, 2021, DOI: 10.1021/acssensors.0c02455
• Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes. Zakashansky, J.A.; Imamura, A.H.; Salgado, D.F.; Romero Mercieca, H.; Aguas, R.F.L.; Lao, A.M.; Pariser, J.; Arroyo-Curras, N.; Khine, M.; Anal. Methods, 2021, DOI: 10.1039/D1AY00041A
• Advances in Nucleic Acid Architectures for Electrochemical Sensing. Wu, Y.; Arroyo-Curras, N.; Curr. Opin. Electrochem., 2021, DOI: 10.1016/j.coelec.2021.100695

2020

• Hydrogel-coating improves the in-vivo stability of electrochemical aptamer-based biosensors. Li, S.; Dai, J.; Zhu, M.; Arroyo-Currás, N.; Li, H.; Wang, Y.; Wang, Q.; Lou, X.; Kippin, T.E.; Wang, S.; Plaxco, K.W.; Li, H.; Xia, F.; bioRxiv, 2020, DOI: 10.1101/2020.11.15.383992
• Real-time monitoring of a protein biomarker. Parolo, C.; Idili, A.; Ortega, G.; Csordas, A. T.; Hsu, A.; Arroyo-Curras, N.; Yang, Q.; Ferguson, B. S.; Wang, J.; Plaxco, K. W.; ACS Sens., 2020, DOI: 10.1021/acssensors.0c01085
• An Electrochemical Biosensor Exploiting Binding-Induced Changes In Electron Transfer Of Electrode-Attached DNA Origami To Detect Hundred Nanometer-Scale Targets. Arroyo-Curras, N.; Sadeia, M.; Ng, A. K.; Fyodorova, Y.; Williams, N.; Afif, T.; Huang, C.; Ogden, N.; Andresen Eguiluz, R.C.; Su, H.; Castro, C. E.; Plaxco, K. W.; Lukeman, P. S.; Nanoscale, 2020, DOI: 10.1039/D0NR00952K
• Hot-SWV: Square Wave Voltammetry with Hot Microelectrodes. Frkonja-Kuczin, A.; Alicea-Salas, J.; Arroyo-Curras, N.; Boika, A.; Anal. Chem., 2020, DOI: 10.1021/acs.analchem.0c00427
• E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum. Parolo, C.; Greenwood, A. S.; Ogden, N. E.; Kang, D.; Hawes, C.; Ortega, G.; Arroyo-Currás, N.; Plaxco, K. W.; Nature Microsyst. Nanoeng., 2020, DOI: 10.1038/s41378-019-0119-5
• Alkanethiol Monolayer End Groups Affect the Long-term Operational Stability and Signaling of Electrochemical, Aptamer-based Sensors in Biological Fluids. Shaver, A; Curtis, S.; Arroyo-Currás, N.; ACS Appl. Mater. Interfaces, 2020, DOI: 10.1021/acsami.9b22385
• From the Beaker to the Body: Translational Challenges for Electrochemical, Aptamer-Based Sensors. Arroyo-Currás, N.; Dauphin-Ducharme, P.; Scida, K.; Chavez, J.L.; Anal. Methods, 2020, DOI: 10.1039/D0AY00026D

2019

• Approaches for the Electrochemical Interrogation of DNA-Based Sensors: A Critical Review. Pellitero, M. A.; Shaver, A.; Arroyo-Currás, N.; J. Electrochem. Soc., 2019, DOI: 10.1149/2.0292003JES
• Discharging Behavior of Confined Bipolar Electrodes: Coupled Electrokinetic and Electrochemical Dynamics. Eden, A.; Scida, K.; Arroyo-Currás, N.; Eijkel, J.C.T.; Meinhart, C.D.; Pennathur, S.; Electrochim. Acta, 2019, DOI: 10.1016/j.electacta.2019.135275
• Electrochemical Aptamer-based Sensors for Improved Therapeutic Drug Monitoring and High-Precision, Feedback-controlled Drug Delivery. Dauphin-Ducharme, P.; Yang, K.; Arroyo-Curras, N.; Ploense, K.L.; Zhang, Y.; Gerson, J.; Kurnik, M.; Kippin, T.E.; Stojanovic, M.N.; Plaxco, K.W.; ACA. Sens., 2019, DOI: 10.1021/acssensors.9b01616
• Open Source Software for the Real-Time Control, Processing, and Visualization of High-Volume Electrochemical Data. Curtis, S.D.; Ploense, K.L.; Kurnik, M.; Ortega, G.; Parolo, C.; Kippin, T.E.; Plaxco, K.W.; Arroyo-Currás, N.; Anal. Chem., 2019, DOI: 10.1021/acs.analchem.9b02553
• Ultra-high-precision, in-vivo pharmacokinetic measurements highlight the need for and a route towards more highly personalized medicine. Vieira, P.A.; Shin, C.; Arroyo-Curras, N.; Ortega, G.; Li, W.; Keller, A.A.; Plaxco, K.W.; Kippin, T.E.; Front. Mol. Biosci., 2019, DOI: 10.3389/fmolb.2019.00069
• Seconds-Resolved Pharmacokinetic Measurements of the Chemotherapeutic Irinotecan In Situ in the Living Body. Idili, A.; Arroyo-Curras, N.; Ploense, K.L.; Csordas, A.T.; Kuwahara, M; Kippin, T.E.; Plaxco, K.W.; Chem. Sci., 2019, DOI: 10.1039/c9sc01495k
• Fluorescence-Based Observation of Transient Electrochemical and Electrokinetic Effects at Nanoconfined Bipolar Electrodes. Scida, K.; Eden, A.; Arroyo-Curras, N.; MacKenzie, S; Satik, Y.; Meinhart, C. D.; Eijkel, J. C. T.; Pennathur, S.; ACS Appl. Mater. Interfaces, 2019, DOI: 10.1021/acsami.9b01339
• Modeling Faradaic Reactions and Electrokinetic Phenomena at a Nanochannel-Confined Bipolar Electrode. Eden, A.; Scida, K.; Arroyo-Curras, N.; Eijkel, J. C. T.; Meinhart, C. D.; Pennathur, S.; J. Phys. Chem. C, 2019, DOI: 10.1021/acs.jpcc.8b10473
• High-precision electrochemical measurements of the guanine-, mismatch- and length-dependence of electron transfer from electrode-bound DNA are consistent with a contact-mediated mechanism. Dauphin-Ducharme, P.; Arroyo-Currás, N.; Plaxco, K.W.; J. Am. Chem. Soc., 2019, DOI: 10.1021/jacs.8b11341

2018

• Surface Attachment Enhances the Thermodynamic Stability of Protein L. Ortega, G.; Kurnik, M.; Dauphin-Ducharme, P.; Li, H.; Arroyo-Currás, N.; Caceres, A.; Plaxco, K.; Angew. Chem., Int. Ed., 2018, DOI: 10.1002/anie.201812231
• High-precision control of plasma drug levels using feedback-controlled dosing. Arroyo-Curras, N.; Ortega, G.; Copp, D.A.; Ploense, K.L.; Plaxco, Z.A.; Kippin, T.E.; Hespanha, J.P.; Plaxco, K.W.; ACS Pharmacol. Transl. Sci., 2018, DOI: 10.1021/acsptsci.8b00033
• Chain Dynamics Limit Electron Transfer from Electrode-Bound, Single-Stranded Oligonucleotides. Dauphin-Ducharme, P.; Arroyo-Curras, N.; Adhikari, R.; Somerson, J.; Ortega, G.; Makarov, D. E.; Plaxco, K. W.; J. Phys. Chem. C, 2018, DOI: 10.1021/acssensors.7b00787

Postdoctoral Work

• Subsecond-Resolved Molecular Measurements in the Living Body Using Chronoamperometrically Interrogated Aptamer-Based Sensors. Arroyo-Curras, N.; Dauphin-Ducharme, P.; Ortega, G.; Ploense, K. L.; Kippin, T. E.; Plaxco, K. W.; ACS Sensors, 2017, DOI: 10.1021/acssensors.7b00787
• High Surface Area Electrodes Generated via Electrochemical Roughening Improve the Signaling of Electrochemical Aptamer-based Biosensors. Arroyo-Curras, N.; Scida, K.; Ploense, K. L.; Kippin, T. E.; Plaxco, K. W.; Anal. Chem., 2017, DOI: 10.1021/acs.analchem.7b02830
• A Simulation-Based Approach to Determining Electron Transfer Rates using Square-Wave Voltammetry. Dauphin-Ducharme, P.; Arroyo-Currás, N.; Kurnik, M.; Ortega, G.; Li, H.; Plaxco, K. W.; Langmuir, 2017, DOI: 10.1021/acs.langmuir.7b00359
• A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors. Li, H.; Dauphin-Ducharme, P.; Arroyo-Currás, N.; Tran, C. H.; Vieira, P. A.; Li, S.; Shin, C.; Somerson, J.; Kippin, T. E.; Plaxco, K. W.; Angew. Chem. Int. Ed. Engl., 2017, DOI:10.1002/anie.201700748
• Real-time measurement of small molecules directly in awake, ambulatory animals. Arroyo-Currás, N.; Somerson, J.; Vieira, P. A.; Ploense, K. L.; Kippin, T. E.; Plaxco, K. W.; PNAS, 2017, DOI: 10.1073/pnas.1613458114
• Dual-reporter drift correction to enhance the performance of electrochemical aptamer-based sensors in whole blood. Li, H.; Arroyo-Curras, N.; Kang, D.; Ricci, F.; Plaxco, K.W.; JACS, 2016, DOI: 10.1021/jacs.6b08671
• Transdermal analyte sensing device. Lansdorp, B.; Strenk, E.; Arroyo-Currás, N.; Imberman, D. U.S. Patent US20160338627 A1(2016)

Graduate School

• Nanometer Scale Scanning Electrochemical Microscopy Instrumentation. Kim, J.; Renault, C.; Nioradze, N.; Arroyo-Currás, N.; Leonard, K.C.; Bard, A.J.; Anal. Chem., 2016, DOI:10.1021/acs.analchem.6b03024.[Link]
• Electrocatalytic Activity of Individual Pt Nanoparticles Studied by Nanoscale Scanning Electrochemical Microscopy. Kim, J.; Renault, C.; Nioradze, N.; Arroyo-Currás, N.; Leonard, K.C.; Bard, A.J.; Anal. Chem., 2016, DOI:10.1021/jacs.6b03980
• Chemical Characteristics of the Products of the Complexation Reaction Between Copper(II) and a Tetra-Aza Macrocycle in the Presence of Chloride Ions. Lincoln, K. M.; Arroyo-Currás, N.; Johnston, H. M.; Hayden, T. D.; Pierce, B. S.; Bhuvanesh, N.; Green, K. N.; J. Coord. Chem., 2015, DOI:10.1080/00958972.2015.1068935
• A Redox Flow Battery that Uses Complexes of Cobalt and Iron with Amino-Alcohol Ligands in Alkaline Electrolytes to Store Electrical Energy. Bard, A. J.; Arroyo-Currás, N.; U.S. Patent, PCT Int. App. WO 2015054260 A2 (2015)
• Iridium Oxidation as Observed by Surface Interrogation Scanning Electrochemical Microscopy. Arroyo-Currás, N.; Bard, A. J.; J. Phys. Chem. C, 2015, 119, 8147-8154
• Development of an Alkaline Redox Flow Battery: From Fundamentals to Benchtop Prototype. Arroyo-Currás, N.; Ph.D. Dissertation, The University of Texas at Austin, June 2015
• An Alkaline Flow Battery Based on the Coordination Chemistry of Iron and Cobalt. Arroyo-Currás, N.; Hall, J.W.; Dick, J.E.; Jones, R.A.; Bard, A.J.; J. Electrochem. Soc., 2015, 162, A378-A383
• Biodegradable Electroactive Polymers for Electrochemically-Triggered Drug Delivery. Hardy, J.G.; Mouser, D.J.; Arroyo-Currás, N.; Geissler, S.; Chow, J.K.; Nguy, L.; Kim, J.M.; Schmidt, C.E.; J. Mater. Chem. B, 2014, 2, 6809-6822
• Electrochemical Monitoring of TiO₂Atomic Layer Deposition by Chronoamperometry and Scanning Electrochemical Microscopy. Satpati, A.K.; Arroyo-Currás, N.; Li, J.; Yu, E.T.; Bard, A.J.; Chem. Mater., 2013, 25, 4165-4172
• Achieving Nanometer Scale Tip-to-Substrate Gaps with Micrometer-Size Ultramicroelectrodes in Scanning Electrochemical Microscopy. Shen, M.; Arroyo-Currás, N.; Bard, A.J.; Anal. Chem., 2011, 83, 9082-9085

Undergraduate School

• Substituent Inductive Effects on the Electrochemical Oxidation of Flavonoids Studied by Square Wave Voltammetry and Ab Initio Calculations. Arroyo-Currás, N.; Rosas-García, V.M.; Videa, M.; Molecules, 2016, DOI:10.3390/molecules21111422
• Comparative Evaluation of a Modified Acetic Method for Extraction of Antioxidant Compounds from Black Beans (Phaseolus vulgaris). Islas, J.F.; Dávalos-Balderas, A.J.; Arroyo-Currás, N.; Cano, B.G.; Galindo-Jacobo, P.; Guajardo-Salinas, G.; Gaytan-Ramos, A., Moreno-Cuevas, J.E.; Food and Nutr. Sci., 2012, 3, 348-353
• Electrochemical Study of Flavonoids in Acetonitrile: Structure-Activity Relationships. Arroyo-Currás, N.; Videa, M.F.; ECS Trans.,2010, 29, 349-359
• Sistema Aprótico para el Estudio Voltamperométrico de Polifenoles: Actividad Antioxidante vs.Conducta Electroquímica. Arroyo-Currás, N.; B.S. Dissertation, ITESM Campus Monterrey, 2009
• Black Bean Extract Ameliorates Liver Fibrosis in Rats with CCl4-induced injury. López-Reyes, A.G.; Arroyo-Currás, N.; Cano, B.G.; Lara-Díaz, V.J.; Guajardo-Salinas, G.E.; Islas, J.F.; Morales-Oyarvide, V.; Morales-Garza, L.A.; Galvez-Gastelum, F.J.; Grijalva, G.; Moreno-Cuevas, J.E.; Ann. Hepatol., 2008, 7, 130-135
• Effects of Bone Marrow Cell Transplant on Thyroid Function in an I131-induced low T4 and elevated TSH rat model. Guajardo-Salinas, G.E.; Carvajal, J.A.; Gaytan-Ramos, A.A.; Arroyo, L.; López-Reyes, A.G.; Islas, J.F.; Cano, B.G.; Arroyo-Currás, N.; Dávalos, A.; Madrid, G.; Moreno-Cuevas, J.E.; J. Negat. Results Biomed.; 2007, 6, 1-8